Preparation of sedimentary organic matter concentrates by microbiological methods
2006
Cvetkovic, O.(Institute of Chemistry, Tehnology and Metallurgy, Belgrade (Serbia). Center of Chemistry) | Vrvic, M.M.(University of Belgrade, Belgrade (Serbia). Faculty of Chemistry) | Dragutinovic, V.(School of Medicine, Belgrade (Serbia). Department of Chemistry) | Vitorovic, D.(Institute of Chemistry, Tehnology and Metallurgy, Belgrade (Serbia). Center of Chemistry)
Investigation on combined bacterial demineralization of sediments i.e. removal of silicates (desilification) by Bacillus circulans and removal of pyrite (depyritization) by Acidithiobacillus ferrooxidans are reviewed in this paper. Using the oil shale from Aleksinac (Serbia) as substrate, the most important aspect of bacterial demineralization was examined: the question of whether or to what extent do the applied bacteria affect the composition and structure of kerogen. High efficiency of Acidithiobacillus ferrooxidans was observed in depyritization experiments (ca 91%). On the other hand, in 30-day-leaching period noticeable elimination of silicates by Bacillus circulans (ca 40%) was achieved. For detailed analyses prior to and after bacterial demineralization, besides standard methods (such as i.r. spectroscopy, elemental microanalysis, micropetrographic analysis), modern analytical instrumental methods were also used (e.g., P-GC and P-GC-MS). The observed results suggested that during these processes the sedimentary organic matter remained native. Thus, the advantage of bacterial vs. chemical demineralization process was demonstrated.
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