Multiple forms of superoxide dismutase in the apoplast and whole-needle extract of Serbian spruce (Picea omorica (Panc.) Purkyne)
2006
Bogdanovic, J.(University of Belgrade, Belgrade (Serbia). Center for Multidisciplinary Studies) | Prodanovic, R.(University of Belgrade, Belgrade (Serbia). Faculty of Chemistry) | Milosavic, N.(Institute of Chemistry, Tehnology and Metallurgy, Belgrade (Serbia). Department of Chemistry) | Prodanovic, O.(University of Belgrade, Belgrade (Serbia). Center for Multidisciplinary Studies) | Radotic, K.(University of Belgrade, Belgrade (Serbia). Center for Multidisciplinary Studies)
Activity and isoenzyme composition of superoxide dismutase in the apoplast and whole-needle extract of Picea omorica (Panc.) Purkyne, was studied. Total SOD activity of the soluble fraction of the needle extract exceeded markedly that of the apoplastic SOD. Several acidic and two slow-migrating basic isoforms were found in the whole extract. Extracellular SOD had an extremely acidic isoform. Using specific inhibitors, we identified Cu/Zn- and MnSOD forms in the total extract, but only MnSOD in the apoplast. The Fe- isoform was not present in a detectable amount.
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