Random amplified polymorphic DNA analysis of Galanga (Alpinia spp.) accessions
2003
Orapin Saritnum(Chiang Mai University, Chiang Mai (Thailand). Faculty of Agriculture. Department of Horticulture) E-mail:[email protected] | Pittaya Sruamsiri(Chiang Mai University, Chiang Mai (Thailand). Faculty of Agriculture. Department of Horticulture)
Thirty-seven galanga (Alpinia spp.) accessions, 31 cultivated and 6 wild landraces from different areas in Thailand were evaluated for genetic diversity, using random amplified polymorphic DNA (RAPD) primers. Out of 22 random primers used in this study, eight primers (OPA20, OPB18, OPC09, OPD02, OPD11, OPG13, OPK12 and OPAX17) produced a total of 73 polymorphic bands. UPGMA cluster analysis of genetic similarity estimates (Jaccards coefficient) separated the accessions into 5 major clusters. The dendrogram showed no relation with their morphological characters such as type, color of rhizome and collection sites which were indicated by the regions of Thailand. However, this study illustrated that RAPD analysis could be a useful tool to evaluate genetic diversity in galanga accessions. The highly- informative primers identified in this study would be available for further genetic analysis of galanga for plant selection and improvement.
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