Full cDNA cloning and tissue distribution of SOCS-2 gene in pig
2007
Du Baowen | Yang Gongshe | Sun Chao
Chinese. 根据Genbank已登录的细胞因子信号转导抑制因子（SOCS-2）基因序列同源区设计1对引物，从8月龄健康雄性八眉猪肾脏组织中提取总RNA，利用RT-PCR技术扩增猪SOCS-2基因；用CLUSTAL W软件进行同源性分析，利用半定量（Semi-quantitative,SQ）RT-PCR技术检测八眉猪各组织中SOCS-2 mRNA的表达量。结果表明，猪SOCS-2基因cDNA全长克隆成功（Genbank登录号为EF121242）；家猪SOCS-2基因与澳洲野猪、人、小鼠、大鼠SOCS-2核苷酸同源性分别为99%，94%，91%，89%，SOCS-2蛋白氨基酸序列同源性分别达到100%，95%，94%，93%；SOCS-2基因在心脏、肺、皮下脂肪组织、腹部脂肪组织、肝脏、脾、肾脏和肌肉组织中广泛表达，其中肾脏组织中最高，心脏次之，肌肉组织中表达量最低。表明猪SOCS-2序列保守性较高，在体内可能具有多种功能。
Show more [+] Less [-]English. SOCS-2 was very important to growth and development of organism and growth hormone signaling. A pair of primers were designed according to the homology region of SOCS-2 among other species. Total RNA was isolated from kidney of Bamei pig, a local strain of Chinese pig, and the complete cDNA sequence of SOCS-2 gene was cloned by RT-PCR technology firstly (Genbank accepted number is EF121242). Thereafter, the distribution of SOCS-2 mRNA in Bamei pig was analyzed by SQ-RT PCR.Compared with others, the homology of SOCS-2 to Sus scrofa, human, mouse and rat was 99%, 94%, 91% and 89%, respectively, but the homology of protein reached 100%, 95%, 94% and 93%, respectively. And the data showed that the SOCS-2 could be detected in many tissues extensively, such as heart, lung, subcutaneous adipose, abdominal adipose, liver, spleen, kidney and muscle. Its expression was higher in kidney and heart but lower in muscle tissue._
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