Application and development of PCR assay to detect Haemophilus parasuis
2007
Yin Xiufeng | Wang Yan | Jiang Ping
Chinese. 通过反应条件的优化、特异性和敏感性的测定,建立了一种快速、简便、准确的PCR方法,用于检测副猪嗜血杆菌(HPs).PCR扩增片段大小为822bp,最小检测量为1080个HPs.与大肠杆菌、沙门氏菌、金黄色葡萄球菌和链球菌无特异性反应.用该法检测人工感染猪的肺脏、心脏和肾脏抽提的DNA,结果为阳性;检测肝脏、脾脏、脑组织样品抽提的DNA,结果为阴性.用该法检测人工感染发病仔猪及自然感染仔猪肺组织,并结合细菌分离培养,证明以肺脏组织为病料,通过细菌分离培养,再用PCR法鉴定分离菌体,可有效地用于HPs的诊断.检测110份来自发病猪场的患病仔猪肺脏病料,其中有53份为阳性,表明HPs所致发的Glasser's病已在我国部分地区发生和流行。
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