Genetic resources of barley and oat characterised by microsatellites
2007
Leisova, L.,Vyzkumny Ustav Rostlinne Vyroby, Prague (Czech Republic) | Kucera, L.,Vyzkumny Ustav Rostlinne Vyroby, Prague (Czech Republic) | Dotlacil, L.,Vyzkumny Ustav Rostlinne Vyroby, Prague (Czech Republic)
The main aim of this study was to evaluate the genetic diversity of barley and oat genotypes within the expert core collections maintained in the RICP Gene bank. Genetic variation of 176 barley accessions was analyzed using 26 microsatellite loci, covering all 6 chromosomes, 330 oat accessions were analyzed using 26 microsatellite loci that are mapped only into linkage groups. For 26 barley microsatellite loci, 328 alleles were detected. The average number of alleles per locus was 12.6. For 26 oat microsatellite loci, 353 alleles were detected. The average number of alleles per locus was 13.6. The average diversity index (DI) was 0.11 in barley and 0.09 in oat. Dendrogram and Principal Component Analysis (PCA) based on microsatellite data showed a different influence of the place of origin, age of variety and pedigree on grouping into clusters. PCA showed that the breeding process had a negative impact on the level of genetic diversity. Therefore, there is a necessity of barley and oat germplasm conservation.
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