Somatic embryogenesis and plant regeneration of Abelmoschus esculentus through suspension culture
2007
Ganesan, M.,Bharathidasan Univ., Tiruchirappalli (India). Dept. of Plant Science | Chandrasekar, R.,Bharathidasan Univ., Tiruchirappalli (India). Dept. of Plant Science | Ranjitha Kumari, B.D.,Bharathidasan Univ., Tiruchirappalli (India). Dept. of Plant Science | Jayabalan, N.,Bharathidasan Univ., Tiruchirappalli (India). Dept. of Plant Science
A simple and reliable protocol for regeneration of okra through somatic embryogenesis from suspension cultures has been developed. Embryogenic callus was obtained from hypocotyl explants cultured on media with Murashige and Skoog (MS) salts, Gamborg (B5) vitamins, 2.0 mg/cubic dm 2,4-dichlorophenoxyacetic acid (2,4-D), 1.0 mg/cubic dm naphthaleneacetic acid (NAA), 25 mg/cubic dm polyvinylpyrrolidone and 30 g/cubic dm sucrose. More number and high frequency of healthy embryoids appeared individually in suspension culture containing MS salts, B5 vitamins, 2.0 mg/cubic dm 2,4-D and 1.0 mg/cubic dm kinetin. Formation of cell clusters from the single cells was clearly noticed during ontogeny. Matured embryos at the cotyledonary stage were transferred to agar solidified medium for germination. The best conversion of embrya into plantlets (67.3 %) was recorded on media with half strength MS salts, B5 vitamins, 0.2 mg/cubic dm benzylaminopurine (BAP) and 0.2 mg/cubic dm gibberellic acid (GA3). The plantlets were transferred to soil and hardened in plastic pots. The regenerated plantlets exhibited all the parental characters without any phenotypic variation.
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