High frequency in vitro propagation of Holarrhena antidysenterica from nodal buds of mature tree
2007
Mallikarjuna, K.,Acharya Nagarjuna Univ., Nagarjuna Nagar (India). Dept. of Botany | Rajendrudu, G.,Acharya Nagarjuna Univ., Nagarjuna Nagar (India). Dept. of Botany
An in vitro method for propagation of Holarrhena antidysenterica has been developed using nodal explants from mature trees growing in the field. Irrespective of concentrations and combinations of growth regulators used, the axillary and terminal buds sprouted and elongated when inoculated on Murashige and Skoog (MS) medium. The highest numbers of shoots were formed when sprouted shoots were subcultured from MS basal medium onto MS medium containing 2 mg/cubic dm N6-benzyladenine (BA) and 0.5 mg/cubic dm alpha-naphthalene acetic acid. The shoot number further increased upon subculture on MS medium containing 0.5 mg/cubic dm BA. By repeated sub-culturing of shoots derived from nodal axillary buds, a high frequency multiplication rate was established. The elongated shoots were excised and rooted in auxin free MS basal medium. Ex vitro rooting of in vitro formed shoots was achieved upon dipping the microshoots for 2 min in 2 mg/cubic dm of indole-3-butyric acid solution. Successful field establishment and high (80-90 %) survival of plants was observed.
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