Effects of oxidized glutathione, bovine serum albumin, cysteine and lycopene on the quality of frozen-thawed ram semen
2007
Uysal, O.,Ankara Univ. (Turkey). Dept. of Reproduction and Artificial Insemination | Bucak, M.N.,Ankara Univ. (Turkey). Dept. of Reproduction and Artificial Insemination
Ram sperm samples were used to determine the effects of the addition of the antioxidants of oxidized glutathione (GSSG), bovine serum albumin (BSA), cysteine and lycopene to freezing media on the post-thawing sperm cells characteristics, including motility, morphology, acrosome integrity, viability and membrane integrity. A total number of 42 ejaculates were collected from 4 Akkaraman rams. Ten replicates of the ejaculates were diluted with a Tris-based extender containing or not containing additives. GSSG (5 mM), BSA (20 mg/mL), cysteine (10 mM) and lycopene (800 microg) showed more positive effects than other concentrations of the supplements and controls in protecting sperm characteristics after the freezing-thawing process (P less than 0.001). The results of this study provide a new approach to the cryopreservation of ram sperm.
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