Incidence of Clostridium perfringens in broiler chickens in the Czech Republic
2007
Svobodova, I.,Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Ustav Hygieny a Technologie Masa | Steinhauserova, I.,Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Ustav Hygieny a Technologie Masa | Nebola, M.,Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Ustav Hygieny a Technologie Masa
The aim of the present study was to detect the incidence of Clostridium perfringens in healthy broiler chickens reared in intensive farms in the Czech Republic. From May 2005 to September 2006, 609 samples of caecal content from broilers (lines Ross 308, Cobb 500) from 23 poultry farms were analyzed. The samples were cultivated on TSC and blood agar, typical colonies were identified and biochemically confirmed. The total number of positive samples was 112 (18.39%). They were processed by PCR for toxin genotyping. The presence of alpha, beta, beta2 and enterotoxin genes was detected. All C. perfringens isolates were classified as type A, four isolates had the cpb2 gene. In conclusion, the prevalence of C. perfringens-positive farms was approximately 74%, the concentration of the pathogen was about 10,000 cfu/g of caecal content.
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