Characterization of inter-varietal chromosome substitution lines of wheat using molecular markers
2008
Pankova, K.,Vyzkumny Ustav Rostlinne Vyroby, Prague (Czech Republic) | Milec, Z.,Vyzkumny Ustav Rostlinne Vyroby, Prague (Czech Republic) | Leverington-Waite, M.,Norwich Research Park (United Kingdom). John Innes Centre | Chebotar, S.,South Plant Biotechnology Centre, Odessa (Ukraine) | Snape, J.W.,Norwich Research Park (United Kingdom). John Innes Centre
Several sets of wheat inter-varietal chromosome substitution lines (SLs) have been produced over the last fifty years at the Crop Research Institute, Prague, Czech Republic, based on cytogenetic manipulations using aneuploids. Lines with defined genes which significantly influence growth habit and flowering time were obtained and used particularly in the study of the genetics and physiology of flowering. The sets of lines include substitutions of homoeologous group 5 chromosomes carrying Vrn genes that control vernalisation response, homoeologous group 2 chromosomes with Ppd genes controlling photoperiodic sensitivity, and some other substitutions, particularly those with chromosome 3B of the Czech alternative variety Ceska Presivka where a novel flowering time effect was located. The use of molecular markers can allow an unambiguous characterization to verify that substitutions are correct and complete. This analysis allowed incorrect substitutions or partial substitutions to be identified and discarded.
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