Characterization of structural proteins of velogenic field isolate (Bom-1) and vaccine strains (R2B and LaSota) of Newcastle disease virus by SDS-Page
2006
Awandkar, S.P. | Majee, S.B. | Sherikar, A.A. | Ghanbahadur, V.S.
Newcastle disease virus(NDV) strains including a velogenic field isolate from Konkan region (BOM-1) and 2 established vaccine strains (R2B and LaSota) were characterized on the basis of electrophoretic mobility of their structral proteins using SDS-PAGE. On virus fragmentation, 3 major protein patterns revealed similarities among all 3 strains of NDV. These proteins were haemagglutinin-neuram inidase (HN-75,000 D), fusion protein (F-66,000 D) and nucleoprotein (NP-56,000 D). The BOM-1 demonstrated 5(205,000 D; 79,000 D; 50,000 D; 44,000 D and 28,000 D) minor viral proteins (VP) while R2B had 8(224,000D; 205,000 D; 98,000D;79,000 D. 50,000 D, 44,000 D, 38,000 D and 28,000 D) and VP79(79,000 D) were common in all 3 strains. The protein profile of BOM-1 revealed differences in the minor proteins whereas structural proteins appeared to be similar to the vaccine strains. The differences in the minor proteins between virulent and vaccine strains reflect the epidemiological significance of the test in molecular characterization of NDV.
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