Isolation Of Rhizoctonia Solani Kأ¼hn The Causal Agent Of Wallflower Foot Rot In Esfahan Greenhouses
2006
Jalali, S., Plant Pests and Diseases Research Dep. , Agricultural Research Center of Esfahan | Emami, M.S., Plant Pests and Diseases Research Dep. , Agricultural Research Center of Esfahan | Ansaripoor, B., Plant Pests and Diseases Research Dep. , Agricultural Research Center of Esfahan
Esfahan province is one of the important places of wallflower (Cheiranthus cheiri L.) production and in recent years is exporting to other provinces such as Tehran, Shiraz and Tabriz. Foot rot and wilting of the plants were obserin wallflower greenhouses. symptoms of the disease in above-ground of infected plants were yellowing, wilting and death of leaves, and crown region shows dark sunken lesions. In order to determine the causal agent of the disease , samples were collected from infected plants and transferred to the lab. Root and crown samples were first thoroughly rinsed with tap water and surface sterilized with 1% sodium hypochlorite. Pieces of stems and roots were cultured on PDA medium and incubated at 27ثڑC . After 2 days,from the majority of the stem pieces,a slow growing fungus was recovered,and isolated by hyphal tip.On the basis of morphological characteristics, the fungus was identified as Rhizoctonia solani Kأ¼hn. As the causal agent of the disease is soilborne and its chemical control in infected soils is difficult, it is proposed to disinfect the soil before use in pots.
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