The Influence Of Accumulation Of Lead On The Contents Chlorophyll, Iron And Calcium In Two Varieties Of Rape Seed (Brassica Napus L.)
2006
Ghorbanli, M., Member of Scientific Board of Plant Physiology, Department of Biology, Gorgan Azad University, Gorgan, Iran | Khanlarian Khatiri, M., Expert in Plant Sciences, | Hajhossini, M., Member of Scientific Board of Biochemistry Department of Biology, Payam Noor University, Tehran Iran, | Zali, H., Member of Scientific Board of Botany, Department of Natural Resources, Mazandaran University, Mazandaran Iran
In this research, the effects of Hoagland, s nutrient with different concentrations of lead (0, 100, 200, 400, ىM Pb( NO3)2 on contents; chlorophylls a, b, total chlorophyll in leaves, ions calcium, iron and Pb2+accumulation in root and shoot of two varieties of rape seed (Brassica napus L.) PF7045.91 and Hyola 401; in hydroponic culture conditions were studied. Samples were digested (wet digstion) using mixture of nitric and perchloric acid was used to contents, ions calcium, iron and lead Concentration of chlorophylls a and b and metals were determined using Spectrophotometer(U.V) model Hitachy U – 2000 and atomic absorption instrument model shimadzo AA – 6300, respectively. In order to interpretation of data, different statistical methods were used. Results showed that, lead caused decrease the contents; chlorophylls a, b and total chlorophyll in leaves and ions Ca2+, Fe2+ in root and shoot of two varieties of rape seed in comparison with control (p 0.05). By increasing lead concentration of nutrient solution, Pb2+accumulation in root and shoot of each two varieties in comparison with control was increased (p 0.05).
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