Purification and identification of alpha sub(s1)- and beta-caseins from ass milk

Vincenzetti, S.; Polidori, P.; Salimei, E.; Mariani, P.L.; Allegri, S.; Cammertoni, N.; Vita, A.

Purification and identification of alpha sub(s1)- and beta-caseins from ass milk

2004

English. In the present study alpha sub(s1)- and beta-caseins were purified from ass milk by gel filtration chromatography followed by anion exchange chromatography on HPLC. The caseins were characterized by sodium dodecyl sulphate-polyacrilamide gel electrophoresis (SDS-PAGE) and, after blotting on PVDF membrane, were identified by N-terminal sequence. The results indicated the presence of beta-caseins with a MW of 35 kDa and 34 kDa and alpha sub(S1)-caseins with a MW of 33 and 30 kDa

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Italian. [In questo studio sono state purificate le alfa inf(s1)- e beta-caseina nel latte di asina mediante cromatografia su gel filtrazione seguita da cromatografia a scambio anionico in HPLC. Le caseine sono state caratterizzate mediante elettroforesi in gel di sodio dodecil solfato-poliacrilamide (SDS-PAGE) e, dopo trasferimento su membrana PVDF, sono state identificate mediante sequenza aminoacidica N-terminale. I risultati hanno indicato la presenza di beta-caseine con un PM di 35 kDa e 34 kDa e di alfa inf(s1)-caseine con un PM di 33 e 30 kDa.]

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AGROVOC Keywords

amino acid sequences asini asno asses bicarbonate bicarbonates bicarbonati bicarbonatos casein caseina chromatographie sur gel cromatografia a scambio ionico cromatografia su gel electroforesis electrophoresis elettroforesi estrazione con solvente extraction par solvant gel chromatography hplc identification identificazione ion exchange chromatography lait latte leche milk ph razze autoctone sequenze aminoacidiche solvent extraction urea uree

Bibliographic information

Published in

Atti della Società Italiana delle Scienze Veterinarie (Italy)

Volume 58 ISSN 1721-1980

Pagination

pp. 1-2

Other Subjects

Cromatografia de cambio ionico; Extraccion por disolventes; Race indigene; Cromatografia sobre gel; Razas indigenas; Chromatographie d'echange ionique; Land races; Secuencias de aminoacidos; Electrophorese; Sequence d'acides amines; Ane; Caseine; Identificacion

Language

Italian

Note

Summary (En)

1 graph

5 ref.

Received 2007

Translated Title

Purificazione e identificazione di alfa inf(s1)- e beta-caseina nel latte di asina

Type

Conference; Summary

Conference

[Italian Society of Veterinary Science. Annual meeting], 58, Grado, Gorizia (Italy), 22-25 Sep 2004

In AGRIS since: 2008-05-15

Format: AGRIS AP

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Purification and identification of alpha sub(s1)- and beta-caseins from ass milk

2004

AGROVOC Keywords

Bibliographic information