An efficient rice variety [Oryza sativa] discrimination system using simple sequence repeat markers
2007
Eshima, A.(Fukuoka-ken. Agricultural Research Center, Chikushino (Japan)) | Wada, T. | Tsubone, M. | Ogata, T.
In order to protect the intellectual property rights of rice varieties, such as the 'Yumetsukushi' and 'Tsukushiroman' varieties that were bred at the Fukuoka Agricultural Research Center, we developed a variety discrimination system using DNA markers. Twelve varieties, which were cultured or distributed in Fukuoka Prefecture, were distinguished from each other using eight SSR markers. The efficient variety discrimination system using SSR markers is composed of two steps. The first step is a bulk analysis, which is carried out as a qualitative analysis, and the second step is a separate analysis that supports the qualitative analysis and gives an estimation of the mixing ratio. In the bulk analysis, the detection limit of Hinohikari's DNA derivative PCR products band on the agarose gel electrophoresis ranged from 5% to 10% when the polished rice and DNA solution of the 'Hinohikari' was mixed with those of the 'Yumetsukushi' at different ratios. We performed a statistical analysis to reveal the essential number of grains at a significance level of 5%. Then, in order to find 10% mixed samples, 29 grains were needed for the bulk analysis, and 80 grains were needed for the separate analysis. Further, to find 20% mixed samples, 14 grains were needed for the bulk analysis, and 20 grains were needed for the separate analysis.
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