Real-Time PCR assay for rapid detection of genus Erysipelothrix DNA from chronic arthritis of erysipelas in swine
2007
Akase, S.(Tokyo-to. Government Office (Japan)) | Miyao, Y. | Sohmura, Y. | Yoda, M. | Suzuki, T. | Imada, Y.
Real-time PCR assay (the TaqMan probe method) was evaluated for the detection of DNA of genus Erysipelothrix directly from chronic arthritic fluids of swine with erysipelas. By using a QIAamp DNA Mini Kit (Qiagen) for DNA extraction, the time frame was less than 4hrs. With this method, in most cases the number of Erysipelothrix organisms in the synovial fluids was estimated to be less than 10E2 cfu per milliliter. The combination of the test results of synovial fluids by enrichment cultivation, real-time PCR and antibody assay by Surface protective antigen (SpaA)-ELISA may give information that is important to understanding the condition of the disease.
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