Influences of surrogate eggshell on viability and hatchability of silkie fowl embryos cultured in vitro and dynamics of minerals in eggshell
2007
Fujita, Y.(Tokyo Univ. of Agriculture (Japan). Faculty of Bioindustry) | Takahashi, S. | Nasu, A. | Shimoi, G. | Kameyama, Y. | Hashizume, R. | Ito, M.
Using White Leghorn eggshell as a surrogate, silkie fowl embryos were cultured in vitro, and its influence on their viability was investigated. In addition, the mineral content of eggshell, which is involved in bone formation, was measured in normal silkie fowl and White Leghorn fertilized eggs on days 0 and 21 of incubation. In in vitro culture, silkie fowl embryos cultured in an incubator by the conventional method for 3 days were transferred into White Leghorn eggshell. The Ca, P, and Mg contents of eggshell of the normal eggs were measured by high-frequency plasma emission spectrochemical analysis on days 0 and 21 of incubation. Using White Leghorn eggshell in in vitro culture, some silkie fowl embryos hatched, but the hatchability was low, and changes in the viability suggested a lack of Ca supply from the eggshell. The content analysis of fertilized eggshell revealed that the Ca reduction rate from day 0 to day 21 was about 1.8 times higher in silkie fowl than in White Leghorn, suggesting a difference in Ca supply between the breeds. However, no major differences were noted in any other mineral content. These findings suggested that the reduction of hatchability in in vitro culture of silkie fowl embryos using White Leghorn eggshell was due to Ca mainly supplied from the eggshell. However, the growth of hatched embryos was normal, and they acquired reproductive ability.
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