Study of the modification of bovine serum albumin and beta-lactoglobulin by Maillard reaction using mono- and di-saccharides
2007
Yajima, K.(Rakuno Gakuen Univ., Ebetsu, Hokkaido (Japan)) | Onodera, S. | Takeda, Y. | Kato, I. | Shiomi, N.
Two proteins, bovine serum albumin (BSA) and beta-lactoglobulin (beta-LG) were modified, by Maillard reaction, with the monosaccharides: glucose or fructose, and the disaccharides: maltose, lactose or xylobiose. The BSA was effectively modified by the reaction with xylobiose at 40degC for 24 h. The results of SDS-PAGE and MALDI TOF-MS analyses have shown that the relative molecular mass (Msub(r)) of the BSA-xylobiose conjugate increased and was estimated to be 8,500. Similarly, the beta-LG was also modified with xylobiose under the same reaction conditions. The Msub(r) of major type of the beta-LG-xylobiose conjugate obtained was estimated to be 21,202 by MALDI TOF-MS analysis. The sugar content was also calculated and was 118 microg/mg of beta-LG-xylobiose conjugate. The available epsilon-amino group of beta-LG decreased by 46.2%. The Maillard reaction also shifted the isoelectric point of the beta-LG to acid side.
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