Culture conditions of psychrotrophic fungus, Penicillium chrysogenum and its lipase characteristics
2007
Cho, H.Y.(University of Southren California, Los Angeles (USA)) | Bancerz, R. | Ginalska, G. | Leonowicz, A. | Cho, N.S. | Ohga, S.
Among 97 fungal strains from the soil collected from the high mountain areas in the Jeju Island, Korea, Penicillium chrysogenum 9 was found to be the best lipase producer. Its lipase productivity reached 42 U/ml in the culture medium. Factors affecting lipase production by Penicillium chrysogenum 9 were studied using fermentation media of different chemical compositions. Under optimal conditions we noted a 1.6-fold increase of lipase activity. The maximum lipase activity was 68 U/ml of culture medium on the 5th day of incubation at pH 6.0 and 20 deg C; therefore, Penicillium chrysogenum 9 was classified as a psychrotrophic microorganism. The enzyme from Penicillium chrysogenum 9 showed a maximum activity at 30 deg C and pH 5.0 for synthetic substrates (triacylglycerols) or at pH 7.0 for natural substrates (oils).
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