In Vitro Bulblet Induction and Plant Regeneration from Immature Zygotic Embryos Cullture of Lilium tsingtauense Gilg.
2006
Ko, J.A. (Chonbuk National University, Jeonju, Republic of Korea) | Kim, H.S. (Rural Development Administration, Suwon, Republic of Korea) | Kim, Y.S. (Chonbuk National University, Jeonju, Republic of Korea)
A system for multiple bulb scale formation and plantlet regeneration from immature embryos explants of Lilium tsingtauense Gilg. cultured on MS medium containing NAA/BA and 2,4-D/BA was established. Developmental stage of embryos and plant growth regulators significantly affected on callus and bulb scale induction. The globular and torpedo stage of embryos did not callus and bulb scale induction. The optimal stage of embryos was early cotyledonary stage of embryos in 70-90 days after pollination. In culture of immature embryos with cotyledonary stage, organogenic callus induced and led to bulb, shoot and root formation.
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