Genetic Diversity of Sweet potato feathery mottle virus from Sweet Potatoes in Korea
2007
Kwak, H.R. (National Institute of Agricultural Science and Technology, RDA, Suwon, Republic of Korea) | Kim, M.K. (National Institute of Agricultural Science and Technology, RDA, Suwon, Republic of Korea) | Jung, M.N. (Mokpo Experiment Station, NICS, RDA, Muan, Republic of Korea) | Lee, S.H. (National Institute of Agricultural Science and Technology, RDA, Suwon, Republic of Korea) | Park, J.W. (National Institute of Agricultural Science and Technology, RDA, Suwon, Republic of Korea) | Kim, K.H. (Seoul National University, Seoul, Republic of Korea) | Ko, S.J. (Jeonnam Agricultural Research and Extension Service, Naju, Republic of Korea) | Choi, H.S. (National Institute of Agricultural Science and Technology, RDA, Suwon, Republic of Korea), E-mail: hschoi@rda.go.kr
Sweet potato feathery mottle virus (SPFMV) is one of the most prevalent viruses infecting sweet potatoes and occurs widely in sweet potato cultivating areas in Korea. To assess their genetic variation, a total of 28 samples infected with SPFMV were subjected to restriction fragment length polymorphism (RFLP) analysis using DNAs amplified by RT-PCR with specific primer sets corresponding to the coat protein (CP) region of the virus. The similarity matrix by UPGMA procedure indicated that 28 samples infected with SPFMV were classified into three groups based on the number and size of DNA fragments by digestion of CP-encoding regions with 7 enzymes including SalI, AluI, EcoRI, HindIII, FokI, Sau3AI, and DraI bands.
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