DNA Sequence Homology Analysis of ars Genes in Arsenic-Resistant Bacteria
2007
Chang, J.S. (Gwangju Institute of Science and Technology, Gwangju, Republic of Korea) | Lee, J.H. (Gwangju Institute of Science and Technology, Gwangju, Republic of Korea) | Kim, K.W. (Gwangju Institute of Science and Technology, Gwangju, Republic of Korea), E-mail: [email protected]
Homology of ars (arsenic-resistance system) genes was examined among the indigenous bacteria isolated from the soils and sediments of two abandoned Au mines, which are highly contaminated with arsenic. The DNA and amino acid sequence homology of the ars determinants were investigated using an ars genotype. The isolated strains showed As(Ⅲ)-oxidation ability contained arsAB genes encoding the efflux pump as well as arsR and arsD regulator genes. The arsR and arsD leader gene are required for an arsenic resistance system when the high-homology genes (arsR; pI258 52.09% and arsD; Shewanell sp. 42.33%) are controlled by the ars inducer-independent regulatory amino acid sequence.
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