Comparative Anatomy of Embryogenic and Non-Embryogenic Calli from Pimpinella brachycarpa
2007
Na, H.Y. (Seoul National University, Seoul, Republic of Korea) | Kim, K.W. (Seoul National University, Seoul, Republic of Korea) | Kwack, Y.R.N. (Seoul National University, Seoul, Republic of Korea) | Kim, S.K. (Seoul National University, Seoul, Republic of Korea) | Chun, C.H. (Seoul National University, Seoul, Republic of Korea), E-mail: [email protected]
Anatomical differences between embryogenic and non-embryogenic calli of Pimpinella brachycarpa were investigated by light microscopy and electron microscopy. Initial callus tissue emerged from explants after 14 d of culturing. The embryogenic calli (EC) were firm, rather opaque, and light yellow in color. The cells usually formed small, compact clusters. Non-embryogenic calli (NEC), however, were friable, semitransparent, and yellow or gray. These formed relatively larger and loosely held clusters.
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