Partial characterization of lectin from the internal organs of black sea cucumber (Holothuria atra Jaeger)
2006
Mojica, E.R.E. | Merca, F.E., Philippines Univ. Los Banos, College, Laguna (Philippines). Inst. of Chemistry
Lectin was extracted from the internal organs of black sea cucumber (Holothuria atra Jaeger) using Tris buffer saline (TBS) pH 7.5 containing 0.15 M NaCl and purified by ammonium sulfate fractionation and Sephadex G-200 gel chromatography. The isolated lectin was non-blood type specific as it agglutinated erythrocytes of all human blood types (A, B, AB and O) and animal erythrocytes (calf, carabao, chicken and goat). Addition of trypsin and calcium ions increased the agglutinating activity of the lectin. Hapten inhibition studies did not show any specificity towards the sugar standards tested. The purified lectin was found to be a glycoprotein as shown by the positive result in Molish test and contained 0.23% total sugar as determined by the phenol-sulfuric acid method. Based on gel chromatography, the estimated molecular weight of the lectin from the internal organs is 335 kD.
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