Adventitious shoot and plantlet formation in medium staple cotton (Gossypium hirsutum L. cv Barac [67]B)
2007
Abdellatef, E. (National Centre for Research, Khartoum (Sudan). Commission for Biotechnology and Genetic Engineering) | Khalafalla, M.M. (National Centre for Research, Khartoum (Sudan). Commission for Biotechnology and Genetic Engineering)
In vitro adventitious shoot regeneration systems are considered most suitable for Agrobacterium- and biolistics-mediated genetic transformation to obtain transgenic plants. In the present investigation, adventitious shoot could be induced directly horn medium staple cotton cotyledonary node devoid of cotyledons and apical meristem. Shoot development was induced on B5 medium supplemented with kinetin (Kin) or benzyladenine (BA) alone or in combination with a naphthalene acetic acid NAA). Both types and concentrations of growth regulators significantly influenced shoot proliferation. Kin proved to be more effective than BA. The best response, however was obtained when 2.0 mg L-1 Kin was used. The efficiency of both BA and Kin for multiple shoot induction was negatively affected when combined with NAA. Elongation of multiple shoots was obtained on half strength agar-solidified B5 basal medium without phytohormones. More than 87% of the in vitro induced shoots produced roots when cultured on half strength B5 medium supplemented with 2.0% sucrose, 0.8% agar and 0.1 mg L-1 NAA. Rooted plants were hardened and 95% survived under greenhouse conditions.
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