Localization specific nucleotide sequences of DNA on rye chromosomes by FISH
2006
Achrem, M.Stanisława M. Rogalska | Rogalska, S.M.,Uniwersytet Szczecinski, Szczecin (Poland). Katedra Biologii Komorki
The phylogenetic relationships of all species genus Secale have been interpreted from different types of data - morphological, ecological, cytogenetic, and hybrid fertility assays. The phylogeny of genus was so far explained by checking the presence, structure, and chromosomal locations of a series of defined repeated sequences making up much of the heterochromatic regions of rye chromosomes (Bedbrook et all. 1980, Jones and Flavel 1982, Cuadrado and Jouve 1997). However, it was difficult to deduce evolutionary trends using only these sequences. The synthetic simple sequence repeats (SSRs) were proven to be useful as cytological markers for identifying chromosomes and for understanding genome organization in plants (Schmidt and Heslop-Harrison 1996, Cuadrado and Schwarzacher 1998, Fuchs et all. 1998, Cuadrado et all. 2000, Cuadrado and Jouve 2002), but SSRs have not been used extensively in phylogenetic studies in rye. In the present work the distribution on rye chromosomes simple sequence repeats (AAC)5 was searched by FISH. These sequence was clustered mainly on the pericentric sites, only small signals were localized at the interstitial sites of the chromosome arm. Distribution of (ACC)5 sequence repeats was specific for each species
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