Fingerprinting of elite cytoplasmic male-sterile (CMS) lines of Pearl millet (Pennisetum glaucum (L.) R. Br.) using proteins and isozymes.
2006
Gulati, S. | Chhabra, A.K. | Behl, R.K. | Nijhawan, D.C. | Dhillon, S.
Seven enzyme systems (amylase [AMY], catalase [CAT], esterase [EST], malic enzyme [ME], peroxidase [PO], phosphoglucoisomerase [PGI] and shikimate dehydrogenase [SKDH]) and seed proteins were used to characterize ten pearl millet CMS lines. Seed protein banding patterns were quite effective in discriminating CMS lines; however, the differentiating bands were mostly of light to very light intensity. Six out of seven enzymes (except CAT) produced one or more unique bands in different CMS lines. PO was the most efficient isozyme among those tested as it distinguished the highest number of CMS pairs, i.e. 43 out of 45 possible comparisons. CAT and ME were the next best enzyme systems, differentiating between 42 of 45 pairs of CMS lines. AMY and EST were moderately effective (each differentiated 34 pairs of CMS lines out of 45 pairs) and PGI was the least effective enzyme system (differentiating only 32 pairs of CMS lines out of 45 pairs). Based on the complementarities of various enzyme systems, we suggest the use of PO in combination with any one of EST, ME, PGI and SKDK to discriminate all ten CMS lines.
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