[Study of mechanism of plant resistance to stresses through transgenesis. Development of information computer technologies to support transgenesis resea]
2007
Kochietov, A.V. | Kolchianov, N.A. | Titov, S.E. | Trifonova, E.A. | Kolodyazhina, Ya.S. | Komarova, M.L. | Sangaev, S.S. | Gerasimova, S.V. | Grogorovichi, D.A. | Levitskij, V.G. | Vishnevskij, O.V. | Ibragimova, S.S. | Smirnova, O.G. | Sapotskij, M.V. | Malinovskij, V.I. | Shumnyj, V.K.
The mechanisms of tobacco plant resistance to viruses and osmotic stress were studied on models of transgenic tobacco plants expressing the gene of secretory ribonuclease fromZinnia elegans (virus resistance) or pyrollin-5-carboxylate synthetase (P5CS) and ornithine aminotrasferase (OAT) genes from Medicago truncatula or two-chain RNA-suppressor of proline dehydrogenase (PDG) gene (osmotic resistance). As is shown, ribonuclease gene transformants were characterized by as high as 7-10 times ribonuclease activity in apoplast, the absence of infection symptoms at a concentration of virus in inoculum 0.1-1 µg/ml and the absence or low content of virus antigen (3.3 – 29.4 against 100-162.4 in control). The transformants expressing P5CS or carrying the gene PDG suppressor were characterized by higher proline content correlating with higher resistance to osmotic stress, salinization and drought. The expression of OAT gene increased the resistance to salinization, but did not raise the content of proline. Higher resistance to heavy metals of plants with suppressed PDG activity has been found. Computer programs have also been created to predict the efficacy of initiation and termination of transgene translation in the cells of grain crops, tomato, tobacco and Arabidopsis. A complex web site (http//wwwmgs.bionet.nsc.ru/mgs/systems/transgene2/) has been developed to support experiments on transgenesis.
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