Comparison and optimization of two PCR tests for identification of Salmonella in poultry feed - stuffs, liver and faeces
2006
Ocepek, M., Ljubljana Univ. (Slovenia). Veterinary Fac. | Pate, M. | Micunovic, J. | Bole-Hribovsek, V.
Classical cultivation method for Salmonella detection is relatively slow, which can sometimes cause serious clinical and economic consequences. The aim of our study was to compare the efficiency of different methods for rapid Salmonella detection in different samples and to optimize the most appropriate method of detection. With the comparison and optimization of two previously described methods we established a new effective method for the rapid detection of Salmonella in animal tissues, faeces and feedstuffs after the enrichment step. This method, including the initial incubation on the nutrient media, was used to detect Salmonellae in the feedstuff samples that contained as low as 2 CFU of Salmonella before the incubation. After DNA extraction with a commercially available DNA extraction kit and after amplification by a previously described nested PCR we were able to find 175 CFU of Salmonella in a tissue sample without pre-incubation.
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