Genetic variation of Triticum turgidum accessions characterized by DNA markers
Kokindova, M.(University of Saint Cyril and Methodius, Trnava (Slovak Republic). Faculty of Natural Sciences | Kraic, J.Research Institute of Plant Production, Piestany (Slovak Republic))
DNA polymorphism at the 14 microsatellite loci was evaluated in 43 accessions of eight subspecies of Triticum turgidum L. Altogether 101 alleles, 53 in the A genome and 48 in the B genome, were detected. The average values of diversity index and polymorphic information content indicate that microsatellites located in the A genome generated higher polymorphism than microsatellites from the B genome. All the analyzed accessions, except two pairs, were different from each other in at least one microsatellite locus. Microsatellite profiles confirmed full identity of all the four analyzed genotypes of ssp. paleocolchicum. Subspecies-specific microsatellite alleles were identified. The average number of alleles in the individual loci indicates a high variation covered by subspecies turgidum, carthlicum, dicoccon, and turanicum. Genotypes of these subspecies can be used in hybridization programmes for extension of genetic variation of intensively bred durum wheats (ssp. durum).
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