Immunoreactivity of rabbits to BLV infection
Revajova, V.(University of Veterinary Medicine, Kosice (Slovak Republic) | Levkut, M. | Levkutova, M. | Balent, P. | Hipikova, V. | Stefkovic, M.
Immune response of rabbits intravenously infected with bovine leukaemia virus (BLV) was observed during 82 weeks. BLV seroconversion of 8 experimental animals examined by AGID assay was observed from 2 to 42 weeks.The examination of lymphocytes showed positivity of BLV provirus in DNA by PCR.Despite BLV antibodies and proving of BLV provirus, complete virus particles were not found out in cultivated lymphocytes with mitogen by transmission electron microscopy.The examinations of leukocytes have shown no significant and lymphocytes significant decrease value of experimental animals from 54 weeks.The evaluation of lymphocyte subsets CD4,CD5,CD11c,CD25 and IgM in peripheral blood demonstrated tendency of IgM positive cells decreasing by flow cytometry in the experimental rabbits.At the end of experiment,examination of the same lymphocyte subpopulations in the spleen of BLV infected rabbits shown increase, except of CD11c,with significance on IgM and CD25 positive cells (P 0.05).Immunohistochemical staining of frozen spleen samples demonstrated more densely popu- lation of IgM,CD4,and CD5 with larger zone of white pulp around periarteriolar lymphoid sheats in BLV infected rabbits in comparison to control animals.
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