Proteomic classification of self-pollinated lines of maize (Zea mays l.) and their single crosses _ reality and perspectives of utilization
2005
Mudry, P.(Trnavska univerzita, Trnava (Slovak Republic). Pedagogicka fakulta, Katedra biologie | Dragun, M.
In this work 18 self pollinated lines (L) of maize (Zea mays L.) and their 13 single crosses (Sc) have been analysed by electrophoretic profiling of 11 enzymes _ acid phosphatase (ACP), alcohol dehydrogenase (ADH), catalase (CAT), diaphorase (DIA), b-glucosidase (GLU), glutamateoxaloacetate transaminase (GOT), isocitrate dehydrogenase (IDH), malate dehydrogenase (MDH), 6-phosphogluconate dehydrogenase (PGD), phosphoglucoisomerase (PGI) and phosphoglucomutase (PGM). All biological materials to be analysed were from breeding programme of SEMPOL Holding (Trnava). From the results it is clear that all samples of biological materials (L and their Sc) were homogeneous. In each from the 13 Sc, male component, female component and their Sc were distinguishable each other by means of this proteomic classification. Eleven from the thirteen Sc and fourteen from the eighteen lines had original fingerprints. Loci: Cat3:9, Dia2:4, Got1:4, Got2:4, Got3:4, Mdh1:6, Mdh4:12, Mmm:M, Pgd2:5 a Pgm1:9 were monomorphic (frequency = 1) and in this set of analysed lines had no discriminatory role. There were detected alleles at individual loci _ Acp1 (alleles 2, 3, 4), Adh1 (4, 6), Cat3 (9), Dia1 (8, 12), Dia2 (4), Glu1 (2, 6-7), Got1 (4), Got2 (4), Got3 (4), Idh1 (4, 6), Idh2 (4, 6), Mdh1 (6), Mdh2 (3, 6), Mdh3 (16, 18), Mdh4 (12), Mdh5 (12, 15), Mmm (M), Pgd1 (2, 3.8), Pgd2 (5), Pgi1 (4, 5), Pgm1 (9) and Pgm2 (1, 4, and 8). New, unique allele/alleles were not identified. In this article aplicability of proteomic classification of maize by enzyme polymorphism as very efficient tool for checking genotypic identity and homogeneity of Slovak maize genotypes for genetic, breeding, seed improvement, ecophysiology and other research purposes are confirmed.
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