Molecular detection and identification of quarantine plant bacteria and the first recorded occurrences in Slovakia
2005
Horvathova, K.(Potato Research and Breeding Inst., Velka Lomnica (Slovak Republic) | Feketova, M. | Horvath, L.
Bacteria Ralstonia solanacearum (RS), Clavibacter michigenensis subsp. sepedonicus (CMS) and Erwinia amylovora (EA), which cause serious bacterial diseases of potatoes and pome fruit trees, are listed as the quarantine plant pathogens in Slovakia and also in other EPPO countries. Their occurence in Slovakia has not been detected up to the last year. In the frame of state research project (1999-2002) development and standardization of DNA-based testing procedures for detection and identification of bacteria in plant samples were carried out. Bacteria RS, CMS and EA were detected by simple or multiplex polymerase chain reaction (PCR) and the specificity of PCR products was confirmed by restriction analysis (RFLP). The first positive detection of RS and CMS in potatoes was recorded in August 2003, the first occurence of EA in pome trees (apple, pear) was recorded in May 2003.
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