Effect of two different antibiotic groups in frozen bovine semen on in vitro produced embryo
2003
Surachit Thongsodsaeng(Department of Livestock Development, Bangkok (Thailand)) | Sumphan Singhachun(Technology Mahanakorn Univ., Bangkok (Thailand))
This study was conducted to compare the effect of two different antibiotic groups, group1 (PS): Penicillin and Streptomycin and groups2 (GTLS): Gentamycin, Tylosin, Lincomycin and Spectinomycin, in bovine frozen semen on in vitro produced embryonic development. Cumulus-oocyte-complexes(COC) were recovered from slaughtered cattle ovaries. Groups of 10 COC each were washed successively 10 times and washed medium was tested for any pathogenic bacteria, Ureaplasma and Mycoplasma contamination. Any groups of COC with positive test result would not be included in statistical analysis. COC were in vitro matured and then were randomly divided into 2 groups. The first group (PS) was inseminated by bovine semen mixed with PS and the second group (GTLS) was inseminated by semen mixed with GTLS. After IVF Embryos were cultured for another 7 days and their developmental competency was assessed. The result indicated that embryos at cleavage stage (48.11 VS 49.05) and morular/blastocystic stage (16.33VS16.48) between PS and GTLS groups, respectively exhibited no significant difference (P GT 0.05).
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