Diagnostic efficiency of Brucella soluble antigens in immunodiffusion tests and ability to differentiate Brucella abortus strain 19 vaccinated cattle

DAFFNER, José; ABALOS, Pedro; PINOCHET, Lautaro; SCORTTI, Mariela; URCELAY, Santiago

Diagnostic efficiency of Brucella soluble antigens in immunodiffusion tests and ability to differentiate Brucella abortus strain 19 vaccinated cattle

1999

DAFFNER, José(University of Chile Faculty of Veterinary and Animal Sciences Department of Preventive Veterinary Medicine) | ABALOS, Pedro(University of Chile Faculty of Veterinary and Animal Sciences Department of Preventive Veterinary Medicine) | PINOCHET, Lautaro(University of Chile Faculty of Veterinary and Animal Sciences Department of Preventive Veterinary Medicine) | SCORTTI, Mariela(University of Chile Faculty of Veterinary and Animal Sciences Department of Preventive Veterinary Medicine) | URCELAY, Santiago(University of Chile Faculty of Veterinary and Animal Sciences Department of Preventive Veterinary Medicine)

English. Three soluble antigens were compared by radial immunodiffusion (RID) and agar gel immunodiffusion (AGID) tests: a native haptene (NH) from Brucella melitensis 16M, and a polysaccharide (PS) from B. abortus 1119-3, both obtained by non-hydrolytic methods, and the (O-Chain) polysaccharide extracted also from B. abortus 1119-3 but using an hydrolytic method. Three groups of bovine sera were tested: a) Naturally infected (n = 76); b) Non-infected (n = 130) and c) S-19 vaccinated (n = 61); the sensitivity (Se), the specificity (Sp) and the ability to differentiate vaccinated (ADV) were determined in each group a, b and c respectively. The highest Se in the RID test (84.3%) was achieved by NH; while the three antigens gave 100% Sp. The O-Chain showed 100% ADV in this test. In the AGID test PS antigen showed the best Se (86.6%), and all antigens showed 100% of Sp and ADV. Finally, for its production qualities and efficiency the antigens PS and NH represent a promising alternative for complementary diagnosis of brucellosis.

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Portuguese. Foram comparados três antígenos solúveis: um hapteno nativo (NH) de B. melitensis 16M, um polissacarídeo (PS) obtido de B. abortus 1119-3 e outro polissacarídeo de cadeia O (O-Chain) originado também da última Brucella. Os testes de imunodifusão radial (RID) e imunodifusão em gel de ágar (AGID) foram confrontados com as três classes de soros bovinos: a) infectados naturalmente (n = 76), b) não infectados (n = 130) e c) vacinados com B19 (n = 61) reagindo a testes sorológicos clássicos. Foram determinadas a sensibilidade (Se), a especificidade (Sp) e a capacidade para discriminar vacinados (ADV). A Se mais alta (84,3%) no teste RID foi demonstrada pelo antígeno NH, enquanto os três antígenos tiveram 100% de Sp. O antígeno O-Chain teve 100% de ADV nesse teste. O teste AGID com estes antígenos demonstrou 100% Sp e ADV, enquanto o antígeno PS mostrou uma melhor Se (86,6%). Finalmente, por sua qualidade de produção e eficiência, os antígenos PS e NH representam uma alternativa segura e econômica para o diagnóstico suplementar da brucelose.

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