Preparation of coupled protein of BSA and recombinant oncosphere antigen TSOL18
2007
Chang Yingzu, Zhao Yuanzhong, Kong Lingfenf
Chinese. 应用异型双功能连接剂MBS(间-马来酰亚胺苯甲酸-N-羟基琥珀酰亚胺酯)将猪带绦虫六钩蚴18ku重组蛋白(TSOL18)和载体蛋白-牛血清白蛋白(BSA)进行连接,制备连接蛋白BSA-TSOL18，用以增强TSOL18的免疫原性。连接蛋白利用快速脱盐柱分离技术进行纯化;纯化后应用醋酸纤维薄膜电泳证明二者的结合情况;并用间接ELISA法检测不同摩尔比连接产物中抗原部分的活性。结果表明:TSOL18与BSA偶连摩尔比为1∶3时连接有效,且对抗原活性影响小，可满足进一步免疫的要求。
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by Institute of Agricultural Information, Chinese Academy of Agricultural Sciences
