Genetic differences in peanut cultivated types (Arachis hypogaea) revealed by SSR polymorphism

Chinese. 本文采用110对SSR引物分析28份花生栽培种资源(7份多粒型、5份龙生型、8份普通型和8份珍珠豆型)间的遗传差异,其中有46对引物在不同品种间检测出2～9个等位基因,多态性信息量(PIC)为0.080～0.869.对28份材料的聚类分析表明,SSR聚类结果与根据形态特征的分类结果基本一致,但在多粒型品种上存在一定差异.标记pPGSseq15C12在本研究中所有的珍珠豆型品种上均扩增出特异性条带,序列分析表明该标记在珍珠豆型与其它类型间扩增片段的差异是由于ATT重复次数不同所致.

English. The 28 accessions of peanut germplasms (Arachis hypogaea L.) were employed to analyze their genetic differences in the cultivated types by using SSR-PCR approach. The tested germplasm would be assigned as four types including fastifgiata type (7 accesions), hirsute type (5 accessions), hypogaea type (8 accessions), and vulgaris type (8 accessions). Forty-six of 110 pairs of SSR primers might detect the polymorphism in the tested peanut germplasms with 2-9 alleles per locus. The value of PIC (polymorphism information content) varied from 0.080 to 0.869. The clustering analysis of these cultivars indicated that the classification of peanut cultivated varieties based on SSR-PCR data would be much more coincident based on traditional morphological classification except the fastifgiata type, it was obviouse that there was difference for identifying the fastifgiata type between the morphological and the SSR molecular criteria. In this research we obtained an SSR marker, pPGSseq15C12, which could generate the specific banding pattern for identifying the peanut vulgaris type. And also the specific band was cloned and sequenced that further showed the genetic differences between vulgaris type and the other types of the tested germplasms resulted from the different repeat number of ATT motif. These results suggested that SSR markers should be high polymorphisms in peanut cultivated genotypes and might be one of the useful tools to reveal the genetic differences at molecular level.