Primary studies on the effects of callus induction and differentiation in Gossypium barbadense
2008
He Yating | Qu Yanying | Kong Qingping
Chinese. 本研究以新疆自育海岛棉品种新海16无菌苗下胚轴为外植体材料,进行愈伤组织诱导、分化及再生植株的初步研究,筛选出适合于海岛棉体细胞胚胎再生的培养体系.结果表明,最佳诱导愈伤组织及分化配方为MSB附加0.1 mg/L 2,4-D及0.05 mg/L KT,分化率达到88%;于MSB1附加0.01 mg/LKT及0.3 mg/L NAA中对胚性愈伤组织进行增值培养;由MSB2附加0.1 mg/L IBA中可分化出成熟体细胞胚胎及再生植株,分化率达73%.本研究通过体细胞胚胎发生途径获得了海岛棉再生植株,为海岛棉遗传转化体系的建立及分子育种奠定了基础.
Show more [+] Less [-]English. Hypocotyl of Xinhai 16, a breed of Gossypium barbadense cultured in Xinjiang, were used as explant to make research on callus induction, differentiation and plant regeneration, in hope of figuring out a favorable culture system for the regeneration of somatic embryo. The result indicates that the best culture medium for callus induction and differentiation is MSB media supplemented with 0.1 mg/L 2,4-D and 0.3 mg/L NAA, plant callus were cultured for production, and then in the MSB2 medium added with 0.1 mg/L IBA, somatic embryo differentiated and plants regenerated, with differentiation rates reaching 73%. In the study, somatic embryogenesis of Gossypium barbadense allows plant to regenerate, thus laying a solid foundation for the establishment of Gossypium barbadense’s genetic transformation and molecular plants breeding.
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