Relationship of H-FABP genotypes with the related productive traits in Hubei white pig and its crossbred

Chinese. 对湖北白猪及其杂交后代H-FABP基因多态性以及与生产性状的相关进行分析，为在湖北白猪优质系培育过程中进行分子标记辅助育种提供理论基础。采用PCR-RFLP（HinfⅠ、HaeⅢ、MspⅠ）方法分析湖北白猪及其杂交后代共计282头猪的H-FABP基因5′-上游区域和第二内含子遗传多态性，利用最小二乘模型分析杂交后代H-FABP基因型对相关生产性能的影响。湖北白猪在HinfⅠ-RFLP位点表现为单态，在HaeⅢ- RFLP和MspⅠ-RFLP位点上呈现多态，杂交一代、杂交二代在三个酶切位点均呈现出多态；湖北白猪在HinfⅠ-RFLP、HaeⅢ-RFLP位点均表现为低度多态，在MspⅠ-RFLP位点表现为中度多态，其杂交一代、杂交二代均表现为中度多态;不同基因型对IMF（Intramuscular fat,肌内脂肪）含量的影响，HH＞Hh＞hh，dd＞Dd＞DD，aa＞Aa＞AA，dd 基因型IMF含量显著高于DD、Dd基因型（P＜0.05），aa、Aa基因型IMF含量极显著高于AA型（P＜0.01）。HH基因型20日龄重显著高于hh基因型（P＜0.05）。其它生产性能在不同基因型间差异不显著（P＞0.05）。在选育群体中可以通过选择H-FABP的优势基因型（aa- dd- HH）提高IMF含量而不影响其它生产性状。

English. The heart fatty acid-binding protein (H-FABP) genotype and its effect on related productive traits were studied in Hubei white pig and its crossbred. These studies were performed in order to provide a theoretial foundation for molecular marker assistant breeding in the breeding proceding of Hubei White High Grade Line. The genetic variation of 5，-Up stream Region and the Second Intron of H-FABP gene in 282 pigs including Hubei white pig and its crossbred were detected by PCR-RFLP with HinfⅠ, HaeⅢ and MspⅠ. Meanwhile, effects of different PCR-RFLP genotypes of H-FABP gene of crossbreed on related productive traits were analyzed by least square analysis. Hubei white pig had no polymorphism at HinfⅠ-RFLP site which had only HH Genotype, but it had polymorphisms at HaeⅢ-RFLP and MspⅠ-RFLP site. Crossbred F1 and F2 had polymorphisms at these three site; Hubei white pig had low polymorphism at HinfⅠ-RFLP and HaeⅢ-RFLP site (PIC＜0.25) while it had mediate polymorphism (0.25＜PIC＜0.5) at MspⅠ-RFLP site. Crossbred F1 and F2 had mediate polymorphism at these three site; The effect of different H-FABP genotype on IMF (Intramuscular fat) content tended to be HHHhhh, dd＞Dd＞DD，aa＞Aa＞AA. IMF content of dd genotype was significantly higher (P＜0.05) than that of DD and Dd genotype at the HaeⅢ-RFLP site. IMF content of aa and Aa genotype was significantly higher (P＜0.01) than that of DD and AA genotype at the MspⅠ-RFLP site. Weight of 20 days of HH genotype was significantly higher (P＜0.05) than that of hh genotype at the HinfⅠ-RFLP site, but groups divided by any other genotypes had no significant differences among other productive performance. Porcine Intramuscular fat content might be improved by increased dominant genotype aa-dd-HH frequency in breeding group, while that did not influence others productive traits.