Construction of Chinese cabbage-pak-choi transcriptome map with cDNA-AFLP techniques
2008
Fan Shuying | Le Jiangang | Cheng Guangjie
Chinese. 构建白菜（Brasscia campestris ssp. chinensis）分子转录图谱。以矮脚黄白菜自交系（97-3-2）和白蔓菁芜菁自交系（001-24），以及以两者为亲本杂交建立的F6重组自交系群体为材料，在莲座期取嫩叶提取RNA并反转录成cDNA，采用cDNA-AFLP技术，利用256对cDNA-AFLP引物对亲本97-3-2、001-24之间的多态性进行检测，筛选出56对多态性引物用于F6群体分析，通过对cDNA-AFLPs分子标记的遗传分析构建图谱。构建了一个包括164个 cDNA-AFLPs 分子标记的分子转录图谱。该图谱由13个连锁群组成，图谱总长度为1 401.2 cM，标记间平均图距9.7 cM。这是第一份利用cDNA-AFLP标记构建的白菜分子转录图谱。
Show more [+] Less [-]English. Chinese cabbage-pak-choi (Brassica campestris L. ssp. chinensis Makino) transcriptome map was constructed with cDNA-AFLP techniques. The inbred line Aijiaohuang 97-3-2, the inbred line Baimanjing 001-24 of turnip [B. campestris ssp. rapifera (Matzg.) Sinsk ] and 183 F6 (recombinant inbred population) plants were used as experimental materials. cDNAs were synthesized from total RNA extracted from young leaves at rosette stage. 256 pairs of cDNA-AFLP primers were used to detect the polymorphisms between parents Aijiaohuang 97-3-2 and Baimanjing 001-24. 56 pairs of cDNA-AFLP primers with high polymorphisms were screened from 256 pairs of primer by DNA-AFLP techniques. The genetic diversity of parents and 183 F6 progenies was detected by 56 pairs of cDNA-AFLP primers. The segregation and distribution of cDNA-AFLPs molecular marker were analyzed to construct transcriptome map among parents and F6 plants. A total of 164 cDNA-AFLPs marker loci were mapped into 13 linkage groups which covered 1 401.2 cM with an average distance of 9.7 cM. It is the first transcriptome map of Chinese cabbage using cDNA-AFLP technique.
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