In vitro regeneration of medicinal plant Centella asiatica
2008
Mohapatra, H.,Utkal Univ., Bhubaneswar (India). Post Graduate Dept. of Botany | Barik, D.P.,Utkal Univ., Bhubaneswar (India). Post Graduate Dept. of Botany | Rath, S.P.,Utkal Univ., Bhubaneswar (India). Post Graduate Dept. of Botany
This paper describes multiple shoot regeneration from leaf and nodal segments of a medicinally important herb Centella asiatica on Murashige and Skoog's (MS) medium supplemented with a range of growth regulators. The highest number of multiple shoots was observed on MS augmented with 3.0 mg/cubic dm N6-benzylaminopurine (BAP) and 0.05 mg/cubic dm alpha-naphthaleneacetic acid (NAA). Leaf explant showed maximum percentage of cultures regenerating shoots (81.6%), with the highest shoot number (8.3 shoots per explant) and the shoot length (2.1 cm), whereas nodal explant showed less number of shoots with callus formation at the base cut end. Successive shoot cultures were established by repeatedly sub-culturing the original explant on a fresh medium. Rooting of in vitro raised shoots was best induced on half strength MS supplemented with 0.5 mg/cubic dm indole-3-butyric acid (IBA) with highest percentage of shoot regenerating roots (76.8%) and 3-4 roots per shoot. Plantlets were acclimated in Vermi-compost and eventually established in soil. Concentrations of chlorophyll, total sugars, reducing sugars and proteins were estimated in leaf tissue from both in vivo and in vitro raised plants. Chlorophyll concentration was higher in in vivo plants, whereas other three components were higher in in vitro plants.
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