Acrosome reaction progress in frozen-thawed and capacitated boar spermatozoa influences the efficiency of in vitro fertilization
2008
Martecikova, S.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic) | Hulinska, P.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic) | Reckova, Z.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic) | Hanzalova, K.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic) | Jeseta, M.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic) | Machatkova, M.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic)
The aim of this study was to monitore the functional status of boar spermatozoa collected from frozen-thawed semen and capacitated in vitro by caffeine. Motile spermatozoa of three boars (A, B and C) were isolated by Percoll gradient, treated with 1mM or 2mM caffeine and incubated in IVF medium or co-incubated with matured porcine oocytes for 3 hours. Motility, viability, acrosome integrity and fertilizing ability of spermatozoa were evaluated. Both motility and viability slightly decreased during capacitation in all tested boars independently of the caffeine treatment (from 50.4+/-8.5 to 38.8+/-5.8 and from 56.0+/-6.9 to 43.9+/-5.5, respectively). The acrosome reaction was faster for 1mM caffeine-treated samples than for 2mM caffeine-treated ones in all sires but its progress was different in each boar. The mean penetration rate was higher for 2mM caffeine-treated samples than for 1mM caffeine-treated ones. On the other hand, monospermy was higher and the mean level of normal fertilization was significantly higher in 1mM caffeine-treated than in 2mM caffeine-treated spermatozoa in all tested boars (29.8+/-6.1 vs. 12.7+/-5.6). The progress of acrosome reaction which was specific for each boar influenced the efficiency of the in vitro fertilization.
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