Relationship between carcass and meat quality traits and polymorphism of CLPS and RYR1 genes in Zlotnicka Spotted pigs
2008
Jankowiak, H.,University of Technology and Life Sciences, Bydgoszcz (Poland). Dept. of Pig Breeding | Kapelanski, W.,University of Technology and Life Sciences, Bydgoszcz (Poland). Dept. of Pig Breeding | Wilkanowska, A.,University of Technology and Life Sciences, Bydgoszcz (Poland). Dept. of Pig Breeding | Jelinska, A.,University of Technology and Life Sciences, Bydgoszcz (Poland). Dept. of Pig Breeding | Biegniewska, M.,University of Technology and Life Sciences, Bydgoszcz (Poland). Dept. of Pig Breeding
The aim of the study was to characterize the polymorphism of CLPS and RYR1 genes in Zlotnicka Spotted breed pigs and to analyse the relation between the CLPS and RYR1 genotypes and carcass and meat quality traits. The study was carried out on 32 fatteners which were slaughtered at approximately 100-110 kg body weight. Genomic DNA was extracted using standard molecular biology methods. Polymorphism in the colipase gene (CLPS) was identified with DdeI restriction enzyme as described by Baskin and Pomp (1998) and RYR1 gene with HinPI according to Fujii et al. (1991). In the studied pig population the allele frequencies of CLPS gene were 0.28 for A and 0.72 for B and those of RYR1 gene 0.75 for N and 0.25 for n. Three genotypes at locus CLPS/DdeI and two genotypes at locus RYR1/HinPI were found. Genotype nn/HinPI was not found. Meat quality traits were determined in the longissimus lumborum muscle. Such traits as the meat pH, colour, drip loss and intramuscular fat content were analyzed. None significant differences in carcass and meat quality traits with respect to CLPS or RYR1 pig genotype effects were found.
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