Determination of cellular fatty acid components in five pathotypes of Sclerospora graminicola, the downy mildew pathogen of Pearl Millet
2002
Geetha, N.P. | Amruthesh, K.N. | Shetty, H.S., University of Mysore, Mysore (India). Department of Applied Botany, Seed Pathology Biotechnology, Downy Mildew Research Laboratory
Sclerospora graminicola, an oomycetous fungus is the incitant of downy mildew disease in pearl millet. Attempts were made to identify the cellular fatty acid composition of S. graminicola pathotypes by employing Gas Chromatography (GC,. Gas chromatography Mass Spectrometry (GCMS, 1D-Proton and 2 D- Hetero Magnetic Quantum Coherence (HMQC- Nuclear Magnetic Resonance (NMR spectra. The pathogen S. graminicola consists of eighteen cellular fatty acids of which the most prevalent saturated fatty acids identified were palmitic (C16:0, heptadecenoic (C17:0 and arachidic(C20:0 acids and the major unsaturated fatty acids wee linoleic (C81:1, linolenic (C18:2, arachidonic (C20:4) and eicosapentaenoic (C20:5 acids. The major cellular fatty acids identified by GC were further confirmed by GC-MS and the unidentified fatty acids were detected by their molecular mass. The saturated fatty acids were caprylic and caproleic acids, and the polyunsaturated fatty acids identified were eicosatrienoic and docosahexaenoic acids. Further, NMR spectra were employed to glean at the fatty acid chains. The fatty acids of S. Graminicola detected by NMR were very low in concentrations and overlapping of significant fatty acid signals were observed. From the studies, the pathogen S. graminicola pathotypes may be identified based on their cellular fatty acid composition as obtained by chromatographic analyses.
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