Total glutathione and glutathione reductase in bovine erythrocytes and liver biopsy
2008
Abd Ellah, M.R.(Iwate Univ., Morioka (Japan). Faculty of Agriculture) | Okada, K. | Goryo, M. | Kobayashi, S. | Oishi, A. | Yasuda, J.
The goal of the present study was to measure the total glutathione level and glutathione reductase activity in bovine erythrocytes and liver biopsy. Five cows were injected intraperitoneally with DL-ethionine (12.5 mg/kg B.W.), and two control cows were injected with normal saline (0.9% NaCl). Ultrasonography guided liver biopsy, and blood samples were collected at 0, 4, 7 and 10 days after injection. The hepatic total glutathione level was significantly increased on days 7 (p0.05) and 10 (p0.01), and hepatic glutathione reductase activity was significantly increased on days 4 (p0.05), 7 (p0.01) and 10 (p0.01). There were insignificant changes in the erythrocytic total glutathione level. The present study demonstrated that liver biopsy is a valuable tool for detecting oxidative stress and for diagnosing hepatic dysfunction in cattle from the viewpoint of the status of glutathione and glutathione reductase.
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