Analysis of deoxynivalenol and nivalenol in cereals by GC-ECD after GPC and silica-gel column cleanup
2005
Ueno, E.(Aichi-ken. Inst. of Public Health, Nagoya (Japan)) | Kabashima, Y. | Oshima, H. | Oka, H.
An efficient gas chromatographic method for determining deoxynivalenol (DON) and nivalenol (NIV) in cereal samples was studied. A macerated sample was extracted with acetonitrile and the acetonitrile layer was separated by salting-out using a graduated cylinder. The extract was cleaned up by selective gel permeation chromatography, and then by a silica-gel column using a disposable pasteur-pipet. A test solution, which was treated with n-trimethylsilylimidazole, and trimethylchlorosilane as catalyst, was subjected to one-injection, dual-column GC with dual ECD (column: Stx-CLPesticides and Stx-CLPesticides2). Detection limits of DON and NIV (0.001 microg/g, both) were found to be applicable to monitoring of these compounds in cereals. Recoveries of DON and NIV from fortified wheat and corn ranged from 81.4 to 90.4% with less than 5% relative standard deviations.
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