Characterization of mulberry mutant growth response to gibberellin and abscisic acid application and its molecular analysis
2009
Sopian, T.(Tokyo Univ. of Agriculture and Technology, Fuchu (Japan)) | Jiao, F. | Hirata, Y.
Characterization of mulberry bending mutant was performed. Mulberry bending mutant 'Garyu' has a relatively short internode compared with the wild genotype 'Ichibei'. The stem bends at the nodal position, and the bending is more apparent in the apical part of the stem. Histological observation showed that the bending occurs from the early developmental stage. Gibberellin (GAsub(3)), natural-type abscisic acid ((S)-(+)-ABA) and their mixture were applied to the winter-dormant stem cutting of 'Garyu' and three normal straight-stem types. Dormancy break and growth of stem cutting was not affected by these hormones in 'Garyu' and 'Shin-ichinose', the hybrid between 'Ichinose' and 'Kokuso 21', but were affected in straight-stem types 'Ichibei' and 'Ryoumenguwa'. Molecular analysis was done by using PCR with GAI degenerate and specific primers producing a fragment which turned to be 765 bp in length after sequencing. The fragment gene has homology to GRAS (GAI, RGA and SCR) transcription factor gene family, which relates to gibberellin response modulation function. We found two substitutive point mutations in GAI-like gene of mulberry 'Garyu' in comparison to mulberry 'Ichibei', 'Shin-ichinose , Kokuso 16', and other GRAS family gene on other plants. These mutations have the consequence to the replacement in the amino acid sequence and might have changed in protein function.
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