The examination to improve the pregnancy rate by the co-transfer of embryo with parthenogenetic activation embryos
2009
Sasaki, K.(Fukui-ken. Animal Experiment Station, Mikuni (Japan)) | Tanaka, K. | Takeuchi, T.
The aim of this study was to improve the pregnancy rate by the co-transfer of embryo with parthenogenetic activation embryo (PA embryo) to secrete a pregnancy signal (interferon tau). Therefore we examined the number of co-transferred PA embryo, the effect of recipient by co-transferred PA embryo and the pregnancy rate of embryo with co-transferred PA embryo. There was no difference between survival rate of the PA embryo frozen with 1.8 M ethyleneglycol (EG) and 1.8 MEG + 0.1 M trehalose as cryoprotectant and the survival rate of the PA embryo was significantly lower than that of in vitro fertilization embryo. We collected it on day 16 after one, two and ten PA embryos transferred at day 7 and investigated the growth situation of the PA embryo, it was not collected from a PA embryo transferred recipients. But PA embryo of 1-15 mm was collected from two PA embryos transferred recipients and ten PA embryos transferred recipients. The return of estrus on one and two PA embryos transferred recipients was investigated, and the rate of a PA embryo transferred recipient extended estrus is 50.0% (4/8) and that of two PA embryos transferred recipient extended estrus is 87.5% (7/8). Extended estrus cycle in recipients two PA embryos transferred was longer than 30.6 days. The pregnancy rate in recipient of embryo with co-transferred PA embryos was 40.0% (8/20) and that of embryo transferred was 38.5% (l0/26) and there was no difference between the former and the latter. On the basis of the results given above, the effect to control corpus luteum degradation was confirmed by PA embryos transfer, but this examination showed there was no difference between the pregnancy rate in recipient of co-transferred embryo with PA embryos and that in recipient embryo transferred.
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