Characterization of transgenic tobacco plants expressing the hvnas1 open reading frame under the control of the AtXyn1 promoter
2009
Higuchi, K.(Tokyo Univ. of Agriculture (Japan). Faculty of Applied Bio-Science) | Hagihara, T. | Mizobuchi, M. | Suenaga, M. | Ito, S. | Saito, A. | Mori, S. | Nishizawa, N.K. | Yoshiba, M.
In order to express nicotianamine (NA) synthase in tobacco plants, particularly in their vascular bundles, we introduced the AtXyn1 promoter::OMEGA-hvnas1 open reading frame cassette into tobacco plants. The accumulation of NA was increased, particularly in the young leaves of transgenic tobacco plants. The Ni tolerance of these plants was slightly higher than that of vector control plants. The Fe content of the whole shoots of the AtXyn1 promoter::OMEGA-hvnas1 transgenic tobacco plants increased under normal conditions regardless of NA contents, compared with the vector control plants.
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